Lens Characterization for Multifocus Microscopy Applications

Multifocus structured illumination microscopy for fast volumetric super-resolution imaging.

We here report for the first time the synergistic implementation of structured illumination microscopy (SIM) and multifocus microscopy (MFM). This imaging modality is designed to alleviate the problem of insufficient volumetric acquisition speed in super-resolution biological imaging. SIM is a wide-field super-resolution technique that allows imaging with visible light beyond the classical diff...

fast multicolor 3 d imaging using aberration - corrected multifocus microscopy

60 | VOL.10 NO.1 | JANUARY 2013 | nature methods fluorescence microscopy using beam splitters to image up to four focal planes on separate cameras3. This method provides excellent light efficiency and a large lateral field of view corresponding to the entire surface area of the camera chip. However, refocusing by translating the camera away from the nominal focal plane induces spherical aberrat...

Assessing fabrication tolerances for a multilevel 2D binary grating for 3D multifocus microscopy.

We perform a comprehensive theoretical assessment of fabrication tolerances for a 2D eight-level binary phase grating that is the central element of a multi-focal plane 3D microscopy apparatus. The fabrication process encompasses a sequence of aligned lithography and etching steps with stringent requirements on layer-to-layer overlay, etch depth and etched sidewall slope, which we show are none...

Preface: Cathode Lens Microscopy for Nanoscience

This issue of the IBM Journal of Research and Development describes several recent advances in the field of nanoscience, with an emphasis on cathode-lens-based microscopy methods, including low-energy electron microscopy (LEEM) and photoemission electron microscopy (PEEM). Also discussed are related analytical approaches such as low-energy electron diffraction (LEED), thermionic electron emissi...

Integrated Device for Circulating Tumor Cell Capture, Characterization, and Lens-Free Microscopy